Fig. 1: GP73 is induced from multiple tissues upon fasting and HFD.
From: GP73 is a glucogenic hormone contributing to SARS-CoV-2-induced hyperglycemia
a,b, Plasma GP73 levels in humans following an overnight fast (a; n = 7) or in mice fed ad libitum (ad-lib) or fasted for the indicated times (b; n = 4). P = 0.0005 for fasted versus fed by two-tailed paired Student’s t-tests (a), P = 0.0180 for fasted (24 h) versus ad-lib by one-way analysis of variance (ANOVA) followed by Bonferroni’s post hoc test (b). c, GP73 mRNA expression in various organs from mice fed ad-lib or fasted for 24 h. BAT, brown adipose tissue; AG, adrenal gland (n = 3). P = 0.0004 for fasted heart versus ad-lib, P = 0.0031 for fasted liver versus ad-lib, P = 0.0012 for fasted kidney versus ad-lib, P = 0.0097 for fasted WAT versus ad-lib, P < 0.0001 for fasted pancreas versus ad-lib by two-tailed Student’s t-tests. d, Representative of three confocal immunofluorescence images of GP73 staining in liver, WAT and pancreatic sections from three mice fed ad libitum or fasted for 24 h. Green represents GP73 and blue represents the nucleus. e, Supernatant GP73 levels in HepG2 cells treated with FSK (20 µM) for the indicated times. P = 0.0018 for FSK versus PBS (48 h), P = 0.0218 for FSK versus PBS (72 h) by two-tailed Student’s t-tests. f,g, Plasma GP73 levels (f) or GP73 mRNA expression in various organs in mice fed a regular diet (reg) or HFD for 4 months (f, n = 4 or 5 in each group; g, n = 3). P < 0.0001 for HFD versus reg (f) by two-tailed Student’s t-tests. P = 0.0010 for fasted heart versus ad-lib, P = 0.0002 for fasted WAT versus ad-lib, P = 0.0135 for fasted liver versus ad-lib by two-tailed Student’s t-tests (g). Animal and cell-based studies were performed independently at least three biological replicates with comparable results. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001.
