Extended Data Fig. 9: SARS-CoV-2 infection promotes GP73 secretion.
From: GP73 is a glucogenic hormone contributing to SARS-CoV-2-induced hyperglycemia
a-b, SARS-CoV-2 genome copy number per μL using specific primers for N (a) or ORF1b (b) in the supernatants of Huh-7 cells infected with SARS-CoV-2 for the indicated times and MOI. c, Cell viability of Huh-7 cells infected with SARS-CoV-2 at 0.1 MOI for the indicated times. d, SARS-CoV-2 viral RNAs in liver and lung tissues from mice inoculated with 1.6 × 104 PFU of MASCp6 and sacrificed at day 2 after inoculation (n = 6). e, Representative of three images of immunofluorescence staining for GP73 and SARS-CoV-2 S in paraffin-embedded lung tissue from three mice inoculated with 1.6 × 104 PFU of MASCp6 and sacrificed at day 3 after inoculation. f, Immunoblotting analysis of GP73 expression in Huh-7 cells transfected with plasmids expressing the indicated SARS-CoV-2 proteins. g, Immunoblotting analysis of SARS-CoV-2 N expression in Huh-7 cells transfected with different concentrations of Flag-N. h, Supernatant GP73 levels in the indicated human cell lines challenged with SARS-CoV-2pp or VSV-Gpp for 24 h. P < 0.0001 for SARS-2-Spp versus VSV-Gpp (HepG2/ACE2), P = 0.0035 for SARS-2-Spp versus VSV-Gpp (A549/ACE2), P = 0.0001 for SARS-2-Spp versus VSV-Gpp (293 T/ACE2), P = 0.0005 for SARS-2-Spp versus VSV-Gpp (Caco-2), P = 0.0002 for SARS-2-Spp versus VSV-Gpp (Huh-7) by two-tailed Student’s t-tests. Animal and cell-based studies were performed independently at least three times with comparable results. The data were the means ± SEMs. **P < 0.01; ***P < 0.001.
