Extended Data Fig. 5: pAAV–CAG-GFP and pAAV-CAG-Rspo2 infection mice on HFD.
From: Identification of a regulatory pathway inhibiting adipogenesis via RSPO2
a–j) Overexpression of RSPO2 by tail vein delivery of AAVs in mice (a). Images of ingWAT and visWAT (b), food intake per day (c), Time-resolved oxygen consumption (d), quantification of triglyceride per gram of liver (e), representative images of H&E staining of liver (f) from pAAV-CAG-GFP and pAAV-CAG-Rspo2 infection mice. Blood glucose (g) and area under curve (AUC) (h) shown by intraperitoneal pyruvate tolerance test. Triglyceride levels in blood (i) and hepatic triglyceride secretion rate (j) after tyloxapol injection. Data shown as mean ± SD (i, j), n = 6 mice. Data analysis was performed using two-tailed Student’s t-test. k–p) Experimental scheme (k) for overexpression of RSPO2 in ingWAT by injection AAV into ingWAT. Quantification of RSPO2 in ingWAT by western blot (l) and in circulation (m). Body weight (n), tissue weight (o), and time-resolved oxygen consumption (p) of pAAV-CAG-GFP and pAAV-CAG-Rspo2 ingWAT infection mice. Data shown as mean ± SD, n = 5 mice (CAG-GFP); n = 6 mice (CAG-Rspo2). Data analysis was performed using two-tailed Student’s t-test. q–r) Spearman correlation of serum RSPO2 level with HOMA-IR (q), mean adipocyte volume (r) in male and female subjects. The correlation coefficient was calculated using a Spearman’s Correlation Test. This figure is related to Fig. 6.
