Extended Data Fig. 7: other markers expression in subpopulations of adipogenitors.
From: Identification of a regulatory pathway inhibiting adipogenesis via RSPO2
a) Scheme for HFD induced obesity. b) eP3 percentage in ingWAT quantified by FACS. Data show as mean ± SD, n = 6 biological replicates. Data was analyzed using two-tailed Student’s t-test. c) Circulating RSPO2 levels in chow and HFD fed mice. Data shown as mean ± SD, n = 6 biological replicates. Data was analyzed using two-tailed Student’s t-test. d) RSPO2 expression quantified by western blot in ingWAT, visWAT and liver. HSP90 served as loading control. Data show the mean ± SD, n = 6 biological replicates. Data was analyzed by two-tailed Student’s t-test. e) Rspo1-4 mRNA levels in Lin-Sca1+ cells and eP3 cells of ingWAT. Data are presented as mean + /- SEM, n = 4 biological replicates. Data was analyzed by one-way ANOVA, in Lin-Sca1+ group, F(3,12)=602, P < 0.0001; in eP3 group, F(3,12)=326, P < 0.0001.
