Fig. 2: Classification of different cell populations within the adipose tissue.
From: Identification of a regulatory pathway inhibiting adipogenesis via RSPO2
a, Flow cytometry dot plots show the new gating strategy used to sort eP1, eP2 and eP3. b, Quantification of adipogenesis (left) and cell number (right) of Lin−Sca1+ cells, eP1 cells, eP2 cells, VAP1+CD142+ APCs, VAP1−CD55− cells, DN:CD142− cells and eP3 induced by A Cocktail (1 μM dexamethasone, 0.5 mM isobutylmethylxanthine and 1 μM insulin). Data are shown as mean ± s.e.m., n = 8 independent wells. Data were analyzed with one-way analysis of variance (ANOVA); F(6,49) = 69.7002, P < 0.0001. c, Microscopy images of different cell populations shown in b on differentiation day 6. Experiment was repeated twice. d, Relative mRNA levels of P1 marker (Cd55, Pcsk6, Efhd1, Pi16 and Smpd3), P2 markers (Vap1, Col4a1, Sparcl1 and Sdc1) and Areg cell-specific marker (Cd142, Gdf10, Igfbp3, Fmo2 and Clec11a) genes in different cell populations; n = 3 biological replicates. Data show mean ± s.e.m. e, Quantification of adipogenesis (left) and cell number (right) of Lin−Sca1+ cells, eP1 cells, eP2 cells and eP3 induced by A Cocktail. Data show mean ± s.e.m.; n = 6 independent wells. Data were analyzed with one-way ANOVA; F(3,20) = 280.8, P < 0.0001 (left); multicomparison with Lin−Sca1+ group was performed by two-stage step-up method with false discovery rate (FDR) = 0.05. F(3,20) = 2.838, P = 0.064 (right). f, Quantification of adipogenesis (left) and cell numbers (right) of Lin−Sca1+ cells, eP1 cells, eP2 cells and eP3 induced by C Cocktail (1 μM insulin). Data are shown as mean ± s.e.m., n = 6 independent wells. Data were analyzed with one-way ANOVA. F(3,20) = 48.27, P < 0.0001 (left), multicomparison with Lin−Sca1+ group was performed by two-stage step-up method with FDR = 0.05. F(3,20) = 0.189, P = 0.903 (right). g, Microscopy images of different cell populations shown in e and f on differentiation day 6. In all panels, nuclei were stained with Hoechst 33342 (blue) and lipids were stained with LD540 (yellow). Scale bars, 100 μm.
