Fig. 4: Rspo2 inhibits adipogenesis of eP1 cells in vivo.
From: Identification of a regulatory pathway inhibiting adipogenesis via RSPO2
a–f, Experimental scheme (a) for cell transplantation in Matrigel. Rspo2 expression in eP1 Matrigel plugs and in eP2 Matrigel plugs (b). Quantification of adipocytes and cell number in eP1 Matrigel plugs (c) and eP2 Matrigel plugs (e). Representative hematoxylin and eosin (H&E) staining of eP1 Matrigel plugs (d) and eP2 Matrigel plugs (f). Data show mean ± s.e.m., n = 3 biological replicates (b), n = 5 biological replicates (c,e). Data analysis was performed using a two-tailed Student’s t-test. Scale bar, 100 μm. g–k, Experimental scheme for overexpression of RSPO2 in AdipoCre-NucRed mice fed with HFD or chow diet. Western blot images (h) and quantification (i) of RSPO2 protein in liver and ingWAT; HSP90 bands were used as loading control. Quantification of adipocyte numbers in ingWAT (j) and visWAT (k) of mice shown in g. Data are shown as mean ± s.d., n = 6 mice. Data analysis was performed by two-tailed Student’s t-test (i) and one-way ANOVA (j,k). In j, Total cell number, F(3,20) = 14.4, P < 0.0001; adipocyte, F(3,20) = 15.50, P < 0.0001; non-adipocyte, F(3,20) = 14.1, P < 0.0001. In k, total cell number, F(3,20) = 14.4, P < 0.0001; adipocyte, F(3,20) = 15.50, P < 0.0001; non-adipocyte, F(3,20) = 14.1, P < 0.0001. l–o, Experimental scheme (l) for overexpression of RSPO2 in ingWAT by injection of AAV into ingWAT of AdipoCre-NucRed mice. Western blot images (m) and quantification (n) of RSPO2 protein in ingWAT of mice shown in l. HSP90 bands were used as loading control. Quantification of cell numbers by quantitative PCR in ingWAT (o). Data shows mean ± s.d., n = 5–6 mice. Data were analyzed using a two-tailed Student’s t-test.
