Fig. 7: snRNA-seq reveals Rspo2 reducing adipocytes number in vivo.

a, Integrated analysis of snRNA-seq, including 14,303 nuclei from ingWAT in mice fed on HFD with chronic expression of GFP or RSPO2 by AAV, yielding 2,218 genes (median). Unsupervised clustering shown as a UMAP plot, seven populations were identified, including adipocytes (adipo) (red), pre-adipocytes (PreA) (blue), macrophages (macro) (green) and natural killer (NK) cells (orange). b, Dot plots for representative markers of each cluster. Expression level (indicated by red color) refers to the log normalized ratio of gene expression reads, normalized to the sum of all reads within each nucleus. Percent expressed refers to the ratio of cells within each cluster that express the genes listed in x axis. c, Cluster compositions in CAG–GFP (n = 7,190 nuclei) and CAG–Rspo2 (n = 7,143 nuclei) conditions. d, Violin plots for Acss2, Nkain2, Sntg1, S100a6, Mrc1 and Gpx1, which are differentially expressed between CAG–GFP and CAG–Rspo2 conditions. e, Subclustering analysis of preadipocyte populations. Unsupervised subclustering of 6,411 preadipocyte nuclei from ingWAT, yielding 2,577 (median) genes. Five subpopulations of preadipocytes (PA-1–PA-5) were identified. f, Feature plots for Dpp4, Pparg and Fmo2, shown as separated plots by conditions. g,h, Pre-adipocyte cluster compositions in CAG–GFP (n = 3,539 nuclei) and CAG–Rspo2 (n = 2,872 nuclei) conditions.