Extended Data Fig. 5: SMA+ and SM22+ mural cells are missing in PgdΔEC mouse embryos. | Nature Metabolism

Extended Data Fig. 5: SMA+ and SM22+ mural cells are missing in PgdΔEC mouse embryos.

From: Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition

Extended Data Fig. 5

a, Sections of E11 control and Pgd𝝙EC mouse embryos immunostained with only secondary antibodies (negative controls) or primary antibodies against α-smooth muscle actin (αSMA) to detect vMC (red) and CD31 for ECs/HCs (green). DAPI (blue) stains all nuclei. D: dorsal, V:ventral, N: notochord. Scale bars: 50μm. b-c, DA sections of E11 control and Pgd𝝙EC mouse embryos immunostained with antibodies against α-smooth muscle actin (αSMA) or SM22 to detect vMC (red) and CD31 for EC/HC (green). DAPI (blue) stains all nuclei. Arrows and arrowheads indicate EC and vMC, respectively. D: dorsal, V: ventral, N: notochord. Scale bars, 20μm. d-e, DA sections of E11 control and Pgd𝝙EC mouse embryos immunostained with antibodies against α-smooth muscle actin (αSMA) (green) and SM22 (red) to detect vMC. DAPI (blue) stains all nuclei. Arrowheads indicate vMC. D: dorsal, V: ventral. Scale bars, 20μm (g) and 10μm (h).

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