Extended Data Fig. 7: ELN regulates adhesion and migration of MC.
a-c, Quantification of ECs area (a), vessel length (b), and branching points (c) per field at the angiogenic front of control and Pgdi𝝙EC pups at P6. Ctrl (n=13) and Pgdi𝝙EC (n=12) from six independent experiments. Data are shown as mean ± SEM. Statistics were done using unpaired Student’s t-test. d, Quantification of phopsho-histone3 (pHH3)-positive cells at the angiogenic front in control and Pgdi𝝙EC pups at P6. pHH3 is used as marker for proliferative EC. Ctrl (n=7) and Pgdi𝝙EC (n=6) from two independent experiments. Data are shown as mean ± SEM. Statistics were done using unpaired Student’s t-test. e, Representative images of transwell migration assay of AoSMCs treated with different concentrations of elastin (ELN) or 600uM R5P overnight. f, Quantification of migrating cells shown in panel e, using ImageJ. Data are shown as mean ± SEM of n=3 from two independent experiments. Statistics were assessed using one-way ANOVA followed by Bonferroni’s multiple comparison test. g, Cell adhesion assay of AoSMCs treated with elastin (ELN) or fibronectin (FN) for 40 min. The percentage of attached cells to total cell inputs was determined using crystal violet staining. Data are shown as mean ± SEM of n=3 from two independent experiments. Statistics were assessed using one-way ANOVA followed by Bonferroni’s multiple comparison test.
