Extended Data Fig. 1: Generation of g6pd and pgd zebrafish mutants.

a, Schematic representation of the zebrafish g6pd gene. Exons are shown as green boxes. In g6pd^sa24272 mutant an ENU-induced C>T nonsense mutation (red) in exon 6 of g6pd leads to a stop codon TAG (* shown in red). In the g6pd gene, the CRISPR targeted sequence is underlined and the protospacer adjacent motif (PAM) sequence is labelled in bold. In g6pd^uto70 mutant the resulting CRISPR-induced rearrangement of 6bp deletion and 20bp insertion in exon 10 (in italic) resulted in a frameshift mutation that leads to premature stop codon at aa 330 (* shown in red). b, Schematic representation of the zebrafish pgd gene. Exons are shown as light blue boxes. In pgd^sa24360 mutant an ENU-induced G>T nonsense mutation (red) in exon 6 of pgd gene leads to a TAA stop codon (*shown in red). In the pgd gene, the CRISPR targeted sequence is underlined and the protospacer adjacent motif (PAM) sequence is labelled in bold. A CRISPR-induced deletion (7 bp) in exon 6 of pgd resulted a frameshift that leads to stop codon at aa 230 (* shown in red). c, Representative sequence chromatograms corresponding to g6pd^wt and g6pd^uto70 animals. The deleted sequence in the mutant is highlighted in blue in the g6pd^wt chromatogram and the inserted sequence in the mutant is highlighted in blue in g6pd^uto70 chromatogram. d, Representative sequence chromatograms corresponding to pgd^wt and pgd^uto71 animals. The sequence deleted in the mutant is highlighted in blue in the pgd^wt chromatogram and the new stop codons in yellow in the pgd^uto71 chromatogram. e, Representative images of the PCR gel from genotyping using genomic DNA from tail fins of adults born from an incross between g6pd^+/uto70- or pgd^+/uto71 heterozygotes. PCR products around 123 bp corresponding to the expected insertion size can be seen in g6pd^uto70 (left) while 113 bp corresponding to the expected deletion size can be seen in pgd^uto71(right).

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