Extended Data Fig. 5: Differentiation of control hiPSCs to generate S6 β-like cells.
From: Abnormal exocrine–endocrine cell cross-talk promotes β-cell dysfunction and loss in MODY8
a, Differentiation protocol used to differentiate hPSCs towards insulin expressing β-like cells. Details are given in Supplementary Table 4. b, Control hiPSCs (family and non-family control) were differentiated to S6 and expression levels of genes specific to β-cells were measured. Two differentiation batches of control hiPSCs (Batch1 n = 4, Batch2 n = 2 biologically independent samples) and human islets collected from five donors were used. Donor information is provided in Supplementary Table 1. β-Actin was used as a housekeeping control. Fold change relative to S0. Data are presented as mean values ± SEM. c, Control hiPSCs (N65-51 family control, n = 2 biologically independent samples) were differentiated to S6 and co-stained for several pancreatic markers; INS/GCG, NKX6.1/PDX1, CHGA/GCG, PDX1/ISL1, NeuroD1/PDX1, NKX2.2/PDX1. Nuclei stained with DAPI (blue). Scale bar is 500 μm.
