Extended Data Fig. 6: Differentiation of gene-edited hESCs and patient-derived hiPSCs to definitive endoderm stage.
From: Abnormal exocrine–endocrine cell cross-talk promotes β-cell dysfunction and loss in MODY8
a, FACS analysis of wild-type and mutant hESC lines differentiated to S1. + / + n = 12, +/1686delT n = 3, +/1698delA n = 3, +/1690_1702del n = 3, +/1683_1704del n = 3, 1686delT/1702delG n = 3 biologically independent samples. Data are presented as mean values ± SEM. No difference was detected in wild-type vs. each mutant line by two-tailed multiple t-test corrected using Holm-Sidak method. b, FACS analysis of control and mutant hiPSC lines differentiated to S1. Control n = 8, Mut+ Dia- n = 6, Mut+ Dia+ n = 7 biologically independent samples. Data are presented as mean values ± SEM. No difference was detected in control vs Mut+ Dia- and vs. Mut+ Dia+ by two-tailed multiple t-test corrected using Holm-Sidak method. c, Immunostaining images, representative of three biologically independent samples, showing DE cells stained for SOX17 (green) and OCT4 (red). Nuclei stained with DAPI (blue). Scale bar is 500 μm.
