Extended Data Fig. 9: Differentiation of gene-edited hESCs and patient-derived hiPSCs to acinar-like organoids.
From: Abnormal exocrine–endocrine cell cross-talk promotes β-cell dysfunction and loss in MODY8
a, Differentiation protocol used to differentiate hPSCs towards exocrine organoids. Details are given in the Methods section. b, Bright field images, representative of three biologically independent samples, show organoids that were derived from gene-edited hESCs. Scale bar is 100 μm. c, Expression levels of genes specific to exocrine pancreas were measured by RT-PCR (WT n = 5, MUT n = 5 biologically independent samples). Data are presented as mean values ± SEM. No difference was detected in wild-type vs. mutant by two-tailed t-test. Similar results were observed using three differentiation batches of hESC or hiPSC lines. β-Actin was used as a housekeeping control. Fold change relative to S0.
