Extended Data Fig. 2: Bcat2 in iWAT protects mice against obesity.
From: BCAA–BCKA axis regulates WAT browning through acetylation of PRDM16
Loss of (a-b) Cre is expressed and Bcat2 is KO in iWAT from injecting AAV-Cre mice but not other organs or AAV-GFP mice, proved by IHC and WB. Bcat2 protein levels as indicated were quantified and normalized against Tubulin. n=2 mice. (c) iWAT-Bcat2 KO prevents HFD-induced weight gain. n=5 mice. (d) Food intake is similar in Bcat2WT and Bcat iWAT-KO mice fed with normal chow food. Representative mean value from 5 mice in a cage. (e) GTT, ITT, and serum markers indicated from WT and Bcat iWAT-KO mice. n=5 mice. (f) iWAT-Bcat2 KO prevents HFD-induced fatty liver. Representative result from 4 mice. (g) Bcat2 KO has no effect on mTORc1 activation. iWAT tissue from overnight starvation mice as a negative control and re-feed as positive control. p-4ebp protein levels as indicated were quantified and normalized against total 4ebp. n=3 mice. Bars and error bars represent mean values and SDs, with biologically individual data points shown. n, cohort size. P values are derived from unpaired, two-tailed t-test.
