Extended Data Fig. 6: Inhibition of γ-secretase activity attenuates muscle atrophy in unloading and diabetic conditions.
From: The endothelial Dll4–muscular Notch2 axis regulates skeletal muscle mass
a,b, Wild-type mice were intraperitoneally injected with a γ-secretase inhibitor DAPT (10 mg kg−1 body weight) or corn oil (Cont) every other day during the HU period. a, Time course. b, Muscle weight of SOL, PLA, and GAS (n = 4 for GC and n = 7 for HU). c–e, Wild-type mice were intraperitoneally injected with STZ (150 mg kg−1 body weight; DM) or vehicle (Veh). Mice were then injected with DAPT or corn oil (Cont) every other day from day 3 following STZ infusion. c, Time course. d,e, Blood glucose levels 14 days after STZ infusion (d) and muscle weight of TA, PLA, and GAS (e) (n = 6 for Veh and n = 8 for DM). Muscle weights were normalised to body weight. Data represent the means ± s.e.m. and points indicate individual mice. *P < 0.05, **P < 0.01, ***P < 0.001, NS (not significant) as determined by: two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test (b,d,e).
