Extended Data Fig. 2: Constitutive activation of Notch2 induces muscle atrophy.

a–g, To examine the effect of constitutive activation of Notch2 on adult muscle in vivo, HSA^rtTA/+;TRE^Cre/+;Rosa26^LSL-N2ICD/+ mice (N2-mTg) were generated. HSA^rtTA/+;TRE^Cre/+ or Rosa26^LSL-N2ICD/+ mice were used as a control (Cont). a, N2-mTg and Cont mice were treated with DOX (2 mg ml⁻¹ in drinking water) to induce constitutive expression of N2ICD. b, qPCR analysis for confirming the overexpression of Notch2 in GAS (n = 3). c,d, Body weight (c) and muscle weights of GAS, TA, and PLA normalised to body weight (d, n = 3 for Cont and n = 5 for N2-mTg). e, Representative images of immunostaining for Laminin on TA cross-sections (scale bar, 100 µm). f, Myofibre CSA of TA (n = 3). g, Grip strength (n = 4 for Cont and n = 5 for N2-mTg). qPCR data were normalised to Tbp expression. Data represent the means ± s.e.m., and points indicate individual mice. *P < 0.05, **P < 0.01, NS as determined by Student’s two-tailed unpaired t-test (c,d,f,g).