Extended Data Fig. 3: Effects of Notch2 ablation on mouse growth and Akita diabetic mice.
From: The endothelial Dll4–muscular Notch2 axis regulates skeletal muscle mass
a, qPCR analysis for confirming the deletion of the Notch2 gene in individual myofibres isolated from EDL muscles of Notch2flox/flox (N2f/f) mice and Mlc1fCre/+;Notch2flox/flox (N2−/−) mice (normalised to Tbp expression). b–e, Profiles of N2–/– adult mice with regard to body weight (b), muscle weights normalised to body weight (c), the number of nuclei per myofibre (d), and the number of Pax7+ cells (satellite cells) per myofibre (e) (n = 5). f,g, Akita mice were used as a diabetic hyperglycemic model. Thirteen-week-old male littermates were used. Blood glucose levels (f) and muscle weights normalised to body weight (g) (n = 3). Data represent the means ± s.e.m. and points indicate individual mice. **P < 0.01, ***P < 0.001, NS (not significant) as determined by: two-way ANOVA followed by Tukey’s multiple comparisons post-hoc test (f,g); Student’s two-tailed unpaired t-test (b–e).
