Extended Data Fig. 4: Antibiotics-treated mice show more severe pulmonary pathology and higher bacillus burdens during M. tuberculosis infection.
(a) Two representative lungs derived from M. tuberculosis-infected mice with antibiotics or water control treatment. Red circles mark the cystic changes, hemorrhage or necrosis on the lungs of infected mice. (b-c) Hematoxylin and eosin (H&E) staining of two representative lungs (b) and the histological scores (c) of M. tuberculosis-infected mice with antibiotics or water control treatment at 5 weeks post infection. Scale bars, 500 μm (top: original magnification), 200 μm (middle: 2.5×) and 50 μm (bottom: 10×). The red-boxed areas at the top are enlarged below. As marked by yellow arrowheads, antibiotics-treated mice showed more severe lesions and more infiltration of inflammatory cells than untreated mice. (d) Quantification analysis of M. tuberculosis CFU in the lung homogenates of M. tuberculosis-infected mice at 5 weeks post infection. (e) The acid-fast staining of M. tuberculosis in lung section of mice at 5 weeks post infection. Note that more acid-fast staining-positive bacilli in lung sections derived from antibiotics-treated mice. Scale bars, 5 μm (top: 100× of original magnification). The red-boxed areas at the top are enlarged below. N = 6 mice per group. Data are representative at least two biological replicates.Data are presented as mean + /- SD. P values were calculated by Student’s two-tailed unpaired t-test [(c) and (d)].
