Extended Data Fig. 1: Healthy controls (HC)and active TB patients (TB) exhibit significant differences in diversity, composition, and abundance of gut bacteria at Shenzhen cohort.
(a) PCoA plot (based on Weighted UniFrac distances). Red points indicated the microbiota enriched in HC (n = 28), blue points indicated the microbiota enriched in TB (n = 26). (b) The relative abundance of gut bacteria at phylum level in the fecal samples from HC (n = 28) and TB (n = 26). (c) The top 10 bacterial species with enriched relative abundance in HC were adjusted P < 0.05 and log2 (HC/TB) > 0, species with enriched relative abundance in TB were adjusted P < 0.05 and log2 (HC/TB) < 0 (HC, n = 28; TB, n = 26). The red box indicates A. muciniphila. (d) The absolute abundance of detected A. muciniphila in the fecal samples from HC (n = 28) and TB (n = 26). (e) Predictive power of top 10 species enriched in HC (that is the top 10 most reduced species in TB) assessed by random forest analysis. Blue boxplots acted as benchmarks. Green boxplots represent confirmed species and red boxplots represent rejected species. Red arrowhead marks A. muciniphila. HC = 28, TB = 26. (f) Histogram of the linear discriminant analysis (LDA) coupled with effect size measurements (LEfSe) identified the species with different abundance between HC and TB. Higher abundant species in TB are shaded with green, higher abundant species in HC are shaded with red. Red arrowhead marks the A. muciniphila, black arrowhead marks B. vulgatus. (g) Circle charts marked the relative abundance of six bacteria with differentiated relative abundance between HC and TB in Shenzhen cohort, and these differentiated six bacteria with differentiated relative abundance were also observed at both Shenzhen and Foshan cohorts. Histogram showed the fold changes of relative abundance of six bacteria, including B. vulgatus, B. uniformis, A. muciniphila, B. caccae, P. merdae, and E. ramosun between HC and TB in Shenzhen cohort (fold changes were calculated as HC/TB). Bacteria are identified by color bars above the chart. Data are presented as mean + /- SD. Pvalues were calculated by PERMANOVA test (a), Mann–Whitney U test [(c) and (d)].
