Extended Data Fig. 3: 1,5-AG inhibited the infections of various coronaviruses.
From: A glucose-like metabolite deficient in diabetes inhibits cellular entry of SARS-CoV-2
(a-c) 1,5-AG presented general inhibitory activity against the entry process of various SARS-CoV-2 variants. (a) The mutation sites in the S2 subunit of the selected SARS-CoV-2 variants with public importance. (b-c) 1,5-AG presented general inhibitory activity against the entry process of various SARS-CoV-2 variants into 293 T/hACE2 (b) and Caco-2 (c) cells. The effectiveness of SARS-CoV-2 spike-mediated viral entry was determined by a luciferase assay (n = 4 biologically independent samples). The gray dotted line represents the value of 50% inhibition rate. (d, e) 1,5-AG inhibited the infections by SARS-CoV and MERS-CoV. (d) Comparison of amino acid sequences for the HR1 peptide of SARS-CoV-2, SARS-CoV and MERS-CoV. Asterisks denote identical amino acid residues among all sequences. V952 and N955 are indicated in red. (e) 1,5-AG presented general inhibitory activity against the entry process of SARS-CoV in 293 T/hACE2 cells (green line) and MERS-CoV in Huh7 cells (blue line). The effectiveness of spike-mediated viral entry was determined by luciferase assay (n = 4 biologically independent samples). The gray dotted line represents the value of 50% inhibition rate. (f-h) Incubation with 1,5-AG did not influence the infection of the IAV H1N1 PR8 strain (f), WSN strain (g) and RSV (h) in the tested cells (n = 4 biologically independent samples). The amount of viral RNA was normalized to human GAPDH. (b-c, e-h) The data are presented as the means±S.E.M. Experiments were performed independently at least three biological replicates with comparable results. (f-h) The data were analyzed using two-tailed Student’s t-test. P values were adjusted using Dunnett’s test to account for multiple comparisons.
