Extended Data Fig. 2: Effects of β-cell-selective deletion of Klb in mice.
From: β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130
a, Daily food intake in KlbF/F, KlbRIP and RIP-Cre mice on STC diet for 12 weeks (n = 5 mice for KlbF/F group, n = 6 mice for KlbRIP group, n = 5 for RIP-Cre group). b-c, Body weight and composition in KlbF/F, KlbRIP and RIP-Cre mice on STC diet for 16 weeks (b, n = 6 mice for each group) or HFD for 12 weeks (c, n = 7 mice for KlbF/F group, n = 7 mice for KlbRIP group, n = 8 mice for RIP-Cre group). d-e. Insulin tolerance test (ITT) in KlbF/F, KlbRIP and RIP-Cre mice on STC feeding for 15 weeks (d) or HFD for 11 weeks (e) (n = 6 mice). f, Representative H&E staining of pancreatic sections of STC- and HFD-fed KlbF/F, KlbRIP and RIP-Cre mice. The islets were circled with blue lines. Similar results were obtained from three independent experiments. Scale bar, 50μm. g, Representative immunofluorescence staining of glucagon (red) and insulin (green) in pancreatic sections of STC- and HFD-fed KlbF/F, KlbRIP and RIP-Cre mice. Similar results were obtained from three independent experiments. Scale bar, 50μm. h, The β-cell mass in STC- and HFD-fed KlbF/F, KlbRIP and RIP-Cre mice (n = 7 mice). i, Relative mRNA levels of β-cell marker genes in islets isolated from STC-fed KlbF/F, KlbRIP and RIP-Cre mice (n = 8 mice). Data are presented as mean ± SEM.
