Extended Data Fig. 3: Restoration of KLB expression alleviates defective GSIS in islets isolated from db/db mice.
From: β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130
a, Representative immunoblots and quantification of KLB in islets isolated from C57BL/6J mice fed with STC or HFD for 16 weeks (n = 8 mice). b, Correlation between GSIS capacity and Klb mRNA level in islets isolated from C57BL/6J mice fed with STC or HFD for 16 weeks (n = 16 mice). c, Representative immunoblots and quantification of KLB in islets isolated from 16-week-old C57BKS m + /db and db/db mice (n = 8 mice). d, Correlation between GSIS capacity and the Klb mRNA level in islets isolated from 16-week-old C57BKS m + /db or db/db mice (n = 16 mice). e, Illustration of the experimental design to evaluate the effect of adenovirus-mediated expression of KLB on insulin secretion in diabetic islets. f, Representative microscopic examination of GFP signals in cultured islets infected with various adenoviruses by paraffin sectioning. Similar results were obtained from three independent experiments. Scale bar, 50μm. g, Representative immunoblots of KLB and Tubulin in islets infected with indicated adenoviruses. Similar results were obtained from three independent experiments. h, Dynamic GSIS and quantification of AUC in islets at 48 h after adenovirus infection (n = 6 mice). i-j, Glycolytic flux measured by 3H2O generated from [5-3H]-glucose (i, n = 8 mice) and ATP production (j, n = 6 mice) in isolated islets infected with indicated adenoviruses. Data are presented as mean ± SEM. P values are derived from two-tailed unpaired t-test with Welch’s correction (a, c), two-tailed nonparametric Spearman correlation (b, d), Welch’s ANOVA followed by Dunnett’s T3 multiple comparisons test (h), or ordinary two-way ANOVA followed by Tukey’s multiple-comparisons test (i, j).
