Extended Data Fig. 4: AAV-mediated depletion of KLB in pancreatic β-cells of adult mice impairs glucose uptake and glycolysis.
From: β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130
Islets were isolated from 16-week-old KlbF/F mice injected with AAV–RIP-GFP or AAV–RIP-Cre, or C57BL/6 J mice injected with AAV–RIP-Cre as described in Fig. 1l. a, Glycolytic flux measured by 3H2O generated from [5-3H]-glucose through glycolysis (n = 8 mice for each group). b, Glucose uptake measured by radioisotope-labeled glucose analog 3H-2-deoxyglucose assay (n = 8 mice for KlbF/F-GFP group, n = 8 mice for KlbF/F-Cre group, n = 7 mice for C57-Cre group). c, Enzymatic activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK) (n = 7 mice for each group). Data are presented as mean ± SEM. P values are derived from ordinary two-way ANOVA followed by Tukey’s multiple-comparisons test (a, c), or ordinary one-way ANOVA followed by Tukey’s multiple-comparisons test (b).
