Extended Data Fig. 8: Overexpression of degradation-resistant GP130 reverses impaired GSIS and glycolysis in KlbRIP islets.
From: β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130
a, Islets isolated from male 16-week-old KlbF/F or KlbRIP mice were infected with adenovirus encoding Flag-tagged GP130 with K716R mutation or GFP at 100 multiplicity of infection (MOI) for 24 h. b, Representative immunoblots with anti-GP130, GFP, Flag or Tubulin antibodies in isolated islets infected with different adenoviruses. Similar results were obtained from three independent experiments. c-d, Insulin secretion (c) and glycolytic flux measured by 3H2O generated from [5-3H]-glucose (d) under 2.8 mM or 16.7 mM glucose conditions in isolated islets infected with different adenoviruses (n = 7 mice for each group). e, Immunoblots for p-STAT3(Tyr705) and t-STAT3 in islets infected with different adenoviruses (upper) and densitometric quantification of the relative expression levels of p-/t-STAT3 (lower, n = 4 mice for each group). f, Relative expression levels of Hif1α and glycolytic genes quantified by real-time PCR and normalized to β-actin gene in islets infected with different adenoviruses. The mRNA level in KlbF/F-GFP group was set as 1 (n = 8 mice for each group). Data are presented as mean ± SEM. P values are derived from ordinary two-way ANOVA followed by Tukey’s multiple-comparisons test (c, d) or ordinary one-way ANOVA followed by Tukey’s multiple-comparisons test (e).
