Extended Data Fig. 1: Loss of TKT in Tregs results in a fatal autoimmune disease.
a, Schematic diagram of two reactions catalyzed by TKT in non-oxidative PPP. G6P: Glucose-6-phosphate; 6PGL: 6-phosphogluconolactone; 6PG: 6-phosphogluconate; Ru5P: Bibulose-5-phosphate; Xu5P: Xylulose-5-phosphate; R5P: Ribose-5-phosphate S7P: Sedoheptulose-7-phosphate; G3P: Glyceraldehyde-3-phosphate; E4P: Erythrose-4-phosphate; F6P: Fructose-6-phosphate; F1,6BP: Fructose 1,6-bisphosphate; DHAP: Dihydroxyacetone phosphate. b, Targeting strategy to generate Treg-specific TKT knockout mice. c, Protein levels of TKT, Foxp3 and β-actin in Treg cells (Tregs) and CD4+CD25-YFP- conventional T cells (Tconvs) sorted from lymphoid tissues of 5-week-old WT or cKO mice. d, Total cell numbers of thymus, spleen and pLN of 5-week-old WT or cKO mice. n=5 mice. e, Flow cytometric analysis and quantification of CXCR5hiPD-1CXCR5hi germinal center follicular helper T (GC-Tfh) cells (gated from TCRβ+CD4+ T cells) in the pLN of 5-week-old WT and cKO mice. n=6 mice. Data are representative of at least two independent experiments. Data are shown as mean ± s.d.. P value are determined by two-way ANOVA followed by Sidak’s multiple-comparisons test (d) and unpaired two-tailed Student’s t-test (e). Numbers in gates indicate percentage of cells.
