Extended Data Fig. 2: Loss of TKT in Tregs disturbs immune homeostasis in multiple organs.

a,b, Flow cytometric analysis of the percentage (a) and number (b) of CD4^–CD8^– (DN), CD4⁺CD8⁺ (DP), CD4⁺CD8^– (SP-CD4) and CD4^–CD8⁺ (SP-CD8) in the thymus as well as CD4⁺ and CD8⁺ T cells in the spleen and pLN of 2-week-old WT and cKO mice. n=5 mice. c-e, Expression of Ki-67 (c), CD62L and CD44 (d,e) in CD4⁺ T cells and CD8⁺ T cells in the spleen and pLN of 2-week-old WT and cKO mice. n=5 mice. f,g, Percentage (f) and cell number (g) of CD4⁺ and CD8⁺ T cells in the pLN from 5-week-old WT and cKO mice. n=6 mice. h,i, Expression of Ki67 in CD4⁺ T cells and CD8⁺ T cells in the spleen (h) and pLN (i) of 5-week-old WT and cKO mice. n=5 mice. j-l, Number or percentage of effector (CD44^hiCD62L^lo) cells in CD4⁺ T cells and CD8⁺ T cells in the spleen (j) and pLN (k,l) of 5-week-old WT and cKO mice. n=6 mice. m,n, IFN-γ, IL-17A and IL-13 productions in CD4⁺ T cells (m) and IFN-γ production in CD8⁺ T cells (n) in the pLN, liver, lung and colon from 5-week-old WT or cKO mice. n=5 mice. Data are representative of at least two independent experiments. Data are shown as mean ± s.d.. P value are determined by two-way ANOVA followed by Sidak’s multiple-comparisons test. ns, not significant.

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