Extended Data Fig. 1: Inactivation of endothelial TAZ mimics several of the YAP/TAZ mutant phenotypes.
From: A YAP/TAZ-TEAD signalling module links endothelial nutrient acquisition to angiogenic growth
a, EdU, ERG and PECAM immunofluorescence labelling of P6 mouse retinas depicting reduced vascular density and impaired endothelial proliferation in Yap/TaziEC-KO (Pdgfb-CreERT2;Yapfl/fl/Tazfl/fl) mice following injection of 4OHT from P1-P4. 4OHT-treated Yapfl/fl/Tazfl/fl mice served as control (Ctrl). A, artery; V, vein. The images of the isolated PECAM signal are shown in grey at lower magnification in the upper part of the panel. b, Confocal images of ERG and PECAM labelled P6 retinas of Ctrl and Yap/TaziEC-KO mice. c, Quantification of vascular parameters in Ctrl and Yap/TaziEC-KO mice at P6 as indicated (EC coverage: n = 12 (Ctrl) and 6 (Yap/TaziEC-KO) independent samples; EC number/field: n = 9 (Ctrl) and 6 (Yap/TaziEC-KO) independent samples; EC proliferation: n = 7 (Ctrl) and 4 (Yap/TaziEC-KO) independent samples). d-f, Confocal images (d,e) and quantifications (f) of PECAM (d) and ERG and PECAM (e) labelled Ctrl (Yapfl/fl) and YapiEC-KO (Pdgfb-CreERT2;Yapfl/fl) retinas at P6 (EC coverage: n = 4 (Ctrl) and 8 (YapiEC-KO) independent samples; EC number/field: n = 4 (Ctrl) and 6 (YapiEC-KO) independent samples). g-i, Confocal images (g,h) and quantifications (i) of PECAM (g) and ERG and PECAM (h) labelled Ctrl (Tazfl/fl) and TaziEC-KO (Pdgfb-CreERT2;Tazfl/fl) retinas at P6 (EC coverage: n = 8 (Ctrl) and 11 (TaziEC-KO) independent samples; EC number/field: n = 5 (Ctrl) and 10 (TaziEC-KO) independent samples). For c, f and i, data represent mean ± s.e.m.; two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ****P < 0.0001; NS, not significant. The numerical data and P values are provided as source data.
