Extended Data Fig. 4: Vascular phenotypes of Tead mutant mice in the postnatal mouse retina.
From: A YAP/TAZ-TEAD signalling module links endothelial nutrient acquisition to angiogenic growth
a-c, PECAM immunofluorescence labelling of P6 (a) Tead2KO (Tead2-/-), (b) Tead4iEC-KO (Pdgfb-CreERT2;Tead4fl/fl) and (c) Tead1/4iEC-KO (Pdgfb-CreERT2;Tead1fl/fl;Tead4fl/fl) retinas, showing only mild vascular phenotypes when compared to controls (Tead2+/+, Tead4fl/fl and Tead1fl/fl;Tead4fl/fl, respectively). d, Quantification of endothelial coverage in Ctrl, Tead2KO, Tead4iEC-KO and Tead1/4iEC-KO mice (EC area: n = 3 (Ctrl) and 4 (Tead2KO); EC area: n = 3 (Ctrl) and 3 (Tead4iEC-KO); EC area: n = 4 (Ctrl) and 4 (Tead1/4iEC-KO) independent samples). e, Higher magnification images of P6 retinas from Ctrl and Tead1/2/4iEC-KO mice, showing a sparse capillary network with clumped endothelial sprouts in the mutants. f, Confocal images of ERG and PECAM stained P6 retinas in Ctrl and Tead1/2/4iEC-KO mice, revealing a hypocellular endothelial network in the endothelial Tead mutant retinas. For d, data represent mean ± s.e.m.; two-tailed unpaired t-test. *P < 0.05; NS, not significant. The numerical data and P values are provided as source data.
