Extended Data Fig. 9: Gating strategy to detect antigen-reactive CD4+ T cells. | Nature Metabolism

Extended Data Fig. 9: Gating strategy to detect antigen-reactive CD4+ T cells.

From: The transcription factor RFX5 coordinates antigen-presenting function and resistance to nutrient stress in synovial macrophages

Extended Data Fig. 9

PBMC were stimulated with Candida albicans antigen (0.05 Units/mL) for 6 days. Antigen-reactive T cells were detected by flow cytometry. a–c, Cells of interest were identified by forward versus side scatter (FSC vs SSC) based on size and granularity (a). Alive cells were identified as 7-AADnegative (b). CD4 + T cells were identified as CD3 + CD4 + cells (c). d, Antigen-reactive CD3 + CD4 + T cells were identified as CD69 + CD40L +. Frequencies in FMO, in non-stimulated cells and in antigen-stimulated cells. e, T cells were primed with antigen as in a–d and restimulated with Mϕ that were loaded with vehicle or antigen. CD3 + CD4 + CD69 + CD40L + T cells were measured after 6 hours.

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