Extended Data Fig. 6: Deletion of p53 in skin adipocytes abrogates UVB-induced appetite enhancement, Related to Fig. 5.
From: Food-seeking behavior is triggered by skin ultraviolet exposure in males
a, Experimental design. b, Melanin pigment accumulation in the mice ears upon indicated treatment (a). c, Weekly mean ears melanin levels of indicated mice upon indicated treatment. (Biologically independent Males: n = 8 mice per condition; Biologically independent females: n = 7 p53flx/flxFabp4Cre- control/UV, p53flx/flxFabp4Cre+ control and n = 8 p53flx/flxFabp4Cre+ UV). d,e, Staircase test for control and p53-cKO (d) male (n = 10 biologically independent mice per condition) and (e) female mice after 5 weeks of daily UVB (50 mJ/cm2) or mock-UVB (control) irradiation. Shown are the measured number of sucrose pellets eaten and of attempts to reach a pellet. (n = 12 biologically independent mice per condition). f, Experimental design. g,h, Upper & middle panels: Number of visits to open and closed arms during 5 min; duration of time spent in the open or closed arms, total distance travelled; velocity; and activity of control and p53-cKO (g) male (n = 12 biologically independent mice per condition) and (h) female mice after 4 weeks of daily UVB (50 mJ/cm2) or mock-UVB (control) irradiation. Lower panel: A representative image of the heat map of the maze for each condition. (n = 11 biologically independent mice per condition). i, Plasma insulin levels in p53-cKO mice after 5 weeks of UVB (50 mJ/cm2) or mock-UVB (control) irradiated control treatment. (n = 9 biologically independent mice per condition). j, Relative mRNA levels of Adiponectin, p21, Ccl2, Tnf, and leptin in the skin adipose tissue of control and p53-cKO male mice after 5 weeks of daily UVB (50 mJ/cm2) or mock-UVB (control) exposure (n = 4 biologically independent mice per condition). Data normalized to 36b4. In all relevant panels: Data are presented as mean ± SEM; Two-tailed unpaired t-test p-values are shown, or statistical details for sex or UVB or p53-cKO factors in the ANOVAs (F-values, degrees of freedom, p-value) with interaction appears in Supplementary Table 10, or two-way ANOVA analysis with multiple correction test appears in Supplementary Table 11.
