Fig. 2: Daily UVB radiation enhances food-seeking behavior in males.
From: Food-seeking behavior is triggered by skin ultraviolet exposure in males
a, Fontana–Masson staining of representative ear sections of mice exposed to daily UVB (50 mJ/cm2) or mock-UVB mice (control). b, Weekly food intake of standard chow food (in grams) during the resting and active phases. (n = 10 biologically independent mice per condition). c, PhenoTyper analysis upon daily UVB (50 mJ/cm2) or mock-UVB (control) for 4 weeks: number of times accessing food (upper panel) or water (lower panel) during the resting and active phases (n = 15 biologically independent male mice per condition; n = 16 biologically independent female mice per condition). d, Weekly mean body weight (grams) after daily UVB (50 mJ/cm2) or mock-UVB (control) irradiation (n = 12 biologically independent mice per condition). e, Respirometry analysis of UVB (50 mJ/cm2) or mock-UVB (control) treated mice 10 weeks after the first UVB treatment. Dot plot represents oxygen flow (ml/min) VO2 consumed at rest (n = 29 for control and n = 28 biologically independent male mice and n = 14 biologically independent female mice per condition). f, Experimental design for g–j. g,h, Staircase test (g) male and (h) female mice (n = 10 biologically independent male per condition and n = 12 biologically independent female mice per condition) (left). Representative images from the video of the test session (right). i,j, Open-field for (i) male and (j) female mice (n = 12 biologically independent mice per condition) (left). Heat maps representative images (right). Far-right: Representative images from the video of the test session. k,l, Mice irradiated daily with UVB (50 mJ/cm2) or mock-UVB (control) for 8 weeks and injected with opioid antagonist (naltrexone (5 mg/kg)) or saline 30 min before the open-field test. Upper panel: Number of sucrose pellets eaten and the total distance travelled by (k) male and (l) female (n = 15 biologically independent male mice per condition and n = 16 biologically independent female mice per condition). Representative heat maps (bottom). In all relevant panels, data are presented as mean ± SEM; Two-tailed unpaired t-test p-values are shown or statistical details for sex or UVB or naltrexone factors in the ANOVAs (F-values, degrees of freedom, p-value) with interaction appears in Supplementary Table 10 or two-way ANOVA analysis with multiple correction test appears in Supplementary Table 11.
