Extended Data Fig. 4: Effects of aspartate on proliferation in the absence of lipids.
From: Cancer cells depend on environmental lipids for proliferation when electron acceptors are limited
a, Proliferation rates of HeLa cells cultured in media +lipids or –lipids, without or with phenformin (100 µM), and/or aspartic acid (10 mM or 20 mM) as indicated (n = 3 per condition from a representative experiment). b, Proliferation rates of HeLa cells cultured in media +lipids or –lipids, without or with phenformin (100 µM), and/or sodium aspartate (10 mM) as indicated (n = 3 per condition from a representative experiment). c, Proliferation rates of H1299 cells cultured in media +lipids or –lipids, without or with phenformin (10 µM), and/or sodium aspartate (10 mM) as indicated (n = 3 per condition from a representative experiment). All bar charts show means with error bars representing ± s.d. Unpaired Student’s t-test was performed where statistics are shown. All experiments were repeated three times or more.
