Extended Data Fig. 5: Effects of exogenous metabolites on ACC phosphorylation.
From: Cancer cells depend on environmental lipids for proliferation when electron acceptors are limited
a, Representative immunoblot of total ACC and ACC serine 79 phosphorylation in HeLa cells cultured for 24 hours in media +lipids or –lipids without or with phenformin (100 µM), pyruvate (1 mM, Pyr), lactate (10 mM, Lac), and/or acetate (200 µM, Ac) as indicated. b, (top) Representative immunoblot of FASN, phosphorylated PDHA (Serine 293), total PDHA, and vinculin in HeLa or H1299 cells overexpressing eGFP or constitutively mature SREBP1a. (bottom) Representative immunoblot of phosphorylated PDHA (Serine 293) and Vinculin in HeLa cultured in –lipids for 24hrs, treated with vehicle, phenformin, antimycin, pyruvate, and/or alpha-ketobutyrate at the indicated doses. All experiments were repeated three times or more.
