Extended Data Fig. 6: Inhibition of mitochondrial electron transport decreases intracellular citrate levels.
From: Cancer cells depend on environmental lipids for proliferation when electron acceptors are limited
a, Relative fractional distribution of citrate isotopomers in HeLa cells cultured for 24 hours in media +lipids or –lipids with U-13C-Glutamine, without and with phenformin (100 µM), pyruvate (1 mM, Pyr), and/or lactate (10 mM, Lac) as indicated (n = 3 per condition from a representative experiment). b, Normalized intracellular ratio of αKG to citrate in HeLa cells cultured in +lipids or –lipids with or without phenformin (100 µM). (n = 6 per condition from a representative experiment). c, Isotopomer distribution of total levels of intracellular citrate in HeLa cells cultured for 24 hours in media +lipids or –lipids with U-13C-Glutamine, without and with phenformin (100 µM), pyruvate (1 mM, Pyr), and/or lactate (10 mM, Lac) as indicated (n = 3 per condition from a representative experiment). All bar charts show means with error bars representing ± s.d. Unpaired Student’s t-test was performed where statistics are shown. All experiments were repeated three times or more. (n = 3 per condition from a representative experiment).
