Extended Data Fig. 7: Bypassing oxidative steps in fatty acid synthesis rescues proliferation in electron acceptor-deficient cells.
From: Cancer cells depend on environmental lipids for proliferation when electron acceptors are limited
a, Proliferation rates of H1299 cells cultured in media +lipids or –lipids without or with phenformin (10 µM) and/or acetate (200 µM) as indicated (n = 3 per condition from a representative experiment). b, Proliferation rates of H1299 cells cultured in media +lipids or –lipids without or with antimycin A (15 nM) and/or acetate (200 µM) as indicated (n = 3 per condition from a representative experiment). c, Relative NAD+ /NADH ratio in H1299 cells cultured in media +lipids or –lipids without or with phenformin (10 µM) and/or acetate (200 µM) as indicated (n = 3 per condition from a representative experiment). d, Proliferation rates of H1299 cells cultured in media +lipids or –lipids in normoxia (21% oxygen), hypoxia (1% oxygen), and/or acetate (200 µM) as indicated. Data from the first four conditions are the same as those presented in Extended Data Fig. 2a. (n = 3 per condition from a representative experiment). All bar charts show means with error bars representing ± s.d. Unpaired Student’s t-test was performed where statistics are shown. All experiments were repeated three times or more.
