Extended Data Fig. 8: Effect of exogenous acetate on levels of TCA cycle intermediates.
From: Cancer cells depend on environmental lipids for proliferation when electron acceptors are limited
a, Relative intracellular alpha-ketoglutarate (αKG) levels in HeLa cells cultured for 24 hours in medium –lipids without or with phenformin (100 µM) and/or acetate (200 µM) as indicated (n = 6 per condition from a representative experiment). b, Relative intracellular succinate levels in HeLa cells cultured for 24 hours in medium –lipids without or with phenformin (100 µM) and/or acetate (200 µM) as indicated (n = 6 per condition from a representative experiment). c, Relative intracellular fumarate levels in HeLa cells for 24 hours in medium –lipids without or with phenformin (100 µM) and/or acetate (200 µM) (n = 6 per condition from a representative experiment). d, Relative intracellular malate levels in HeLa cells cultured for 24 hours in medium –lipids without or with phenformin (100 µM) and/or acetate (200 µM) (n = 6 per condition from a representative experiment). All bar charts show means with error bars representing ± s.d. Unpaired Student’s t-test was performed where statistics are shown. All experiments were repeated three times or more.
