Extended Data Fig. 1: Effects of lipid depletion and fatty acid synthesis inhibition on cell proliferation.

a, Cell culture media was prepared with delipidated serum, and then reconstituted with 1% Lipid Mixture (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic acid, and 0.22 mg/ml cholesterol) (+lipids) or vehicle (–lipids). Proliferation rates of HeLa cells cultured in media +lipids or –lipids without and with the FASN inhibitor GSK2194069 (0.3 µM) as indicated (n = 3 per condition from a representative experiment). b, Relative palmitate synthesis rates of HeLa cells cultured in media +lipids or –lipids without and with GSK2194069 (0.3 µM), or without and with phenformin (100 µM) as indicated (n = 3 per condition from a representative experiment). c, Oxygen consumption rate (OCR) of HeLa cells cultured in media +lipids or –lipids as indicated (n = 10 per condition from a representative experiment). d, OCR of H1299 cells cultured in media +lipids or –lipids as indicated (n = 10 per condition from a representative experiment). e, OCR of H1299 cells cultured in –lipid and acutely treated with 1% Lipid Mixture or Tween-80 and Pluronic F-68 equivalent to what is present in 1% Lipid Mixture (n = 10 per condition from a representative experiment). f, OCR of HeLa cell cultures in media +lipids or –lipids acutely treated with the SCD1 inhibitor A939572 (1 µM) and rotenone (1.5 µM) + antimycin A (1.5 µM) as indicated (n = 8 per condition from a representative experiment). All bar charts and line graphs show means with error bars representing ± s.d. Unpaired Student’s t-test was performed where statistics are shown. All experiments were repeated three times or more.

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