Extended Data Fig. 6: FGF21 is increased in pups but not mothers after prolonged breastfeeding.
From: Prolonged breastfeeding protects from obesity by hypothalamic action of hepatic FGF21
The different levels observed in FGF21 between SW-HFD and DW-HFD animals are not coming from the mothers. a–c, Effects of delayed weaning on mother´s: body weight (a), plasma FGF21 levels (b), milk FGF21 levels. d–f, Effects of delayed weaning on pups: liver protein levels of FGF21 (n = 9–10) (d); BAT protein levels of FGF21 (e); plasma NEFAS (f). Protein data were expressed in relation (%) to control (SW) animals. β-actin was used to normalize protein levels. Dividing lines indicate splicing within the same gel. Values are represented as means ± SEM, n per group indicated in each figure. Exact P values are shown. Statistical differences according to a two-sided Student’s t-test (normal data) (a, b, c, d, e and f).
