Extended Data Fig. 10: D2R signalling in LHA/ZI GABA neurons is required for FGF21 action.
From: Prolonged breastfeeding protects from obesity by hypothalamic action of hepatic FGF21
a,b, Fluorescent activated cell sorting (FACS) to isolate D2R-expressing neurons (GFP-positive) in the LHA/ZI (a); FGF21 receptor (FGFR1) mRNA is expressed in isolated D2R cells from the LHA/ZI (b). c,d, Effect of injecting an adenoviral vector encoding a scrambled RNA (Ad-hSyn-DIO-EGFP) or an shRNA against D2R (Ad-hSyn-DIO-shD2r-EGFP) in a Cre-dependent manner, followed by ICV injection of vehicle or FGF21 in Vgat-ires-Cre mice on body weight (c) and quantification of immunolabeling for UCP1 in BAT (d). e, Photomicrograph showing the colocalization of Vimentin and GFP in the lateral ventricle.
