Extended Data Fig. 2: Aged blood decreases muscle strength in young mice.
From: Systemic induction of senescence in young mice after single heterochronic blood exchange
(a) Twitch force generated by skeletal muscles and maximal rate of contraction and relaxation during contractions (n = 6 for YY; n = 8 for YO). (b) Representative images of Oil Red O staining and quantification of Oil Red O + area of skeletal muscles of young mice receiving old (YO; 4-10 images per mice / n = 5 mice) or young (YY; 3-5 images per mice / n = 6 mice) mouse blood, showing more accumulation of adipose tissues in endomysium (interstitial connective tissue) between fibers in YO mice and (c) percentage of fibrosis (n = 4 for YY; n = 5 mice; 3-4 images per mice). (d) Skeletal muscle fatigue assessment in YY and YO mice (n = 6 for YY and n = 5 for YO). (e) Running distance in meter of YY and YO on treadmill (n = 11 for YY and n = 8 for YO). (f) Latency time to fall off the rotarod as a measure of motor coordination. Data are means ± s.e.m. of biologically independent samples. A two-tailed Student t-test (a) and two-tailed t-test with a Welch’s correction (b-c, e-f) with *, P < 0.05; **, P < 0.01were used for statistical analysis. Scale bars are shown in each image.
