Extended Data Fig. 4: Metabolic dynamics measurements on mouse kidney.
From: Analyzing cell-type-specific dynamics of metabolism in kidney repair
a, Pseudo-images showing the 13C enrichment of isotopologues of different metabolites over a time course (up to 2 h) of incubation with U-13C6-glucose. Color scale representing the 13C enrichment (%). Molecular histology and immunofluorescent staining on post-MALDI-MSI tissue are shown in Fig. 3a. b, Pseudo-images showing the 13C enrichment of isotopologues of different metabolites during incubation with U-13C5-glutamine for 2 h. Color scale representing the 13C enrichment (%). c, Pseudo-images showing the 13C enrichment of Glu M + 2 isotopologues during incubation with U-13C18-linoleate for 2 h. Color scale representing the 13C enrichment (%). d-f, Graphs showing the curve of 13C enrichment of isotopologues of metabolites over a time course (up to 2 h) with incubation of U-13C6-glucose (d), U-13C5-glutamine (e) and U-13C18-linoleate (f). Average values of all pixels and values from two representative pixels are shown. Images show the average 13C enrichment (area under curve (AUC) normalized to total time) of isotopologues measured at different timepoints. All scale bars = 200 µm. Abbreviations: 3PG, 3-phosphoglycerate; G3P, glycerol 3-phosphate; α-KG, alpha-ketoglutaric acid.
