Extended Data Fig. 1: Cholesterol promotes HCC development by SNHG6. | Nature Metabolism

Extended Data Fig. 1: Cholesterol promotes HCC development by SNHG6.

From: Long non-coding RNA SNHG6 couples cholesterol sensing with mTORC1 activation in hepatocellular carcinoma

Extended Data Fig. 1

(A) Analyzing SNHG6 expression in liver cancer datasets. The horizontal line inside the box is the median; the top is the upper quartile; the bottom is the lower quartile; the upper whiskers extend from the upper quartile to the highest actual value; the lower whiskers extend from the lower quartile The number is extended to the lowest actual value. Two-sided Student’s t test. (B) Overall Survival analysis of SNHG6 in the liver cancer database. Kaplan-Meier analysis. (C) MTT assay of Vector-/SNHG6-Huh7 cells. Data are mean values ± S.D. Two-way ANOVA. (D-E) Colony formation of sgCtrl /sgSNHG6 HepG2 cells (D) and of shScr/shSNHG6 Huh7 cells (E). Data are mean values ± S.D. One-way ANOVA with Tukey’s test. (F-G) qRT-PCR detection of 12 candidates’ expression upon fatty acid stimulation (0.4 mM palmitic acid, 24 h) (F); glucose stimulation (24 h) (G). Data are presented as mean values ± S.D. Two-sided Student’s t-test. (H) qRT-PCR detection of SNHG6 expression at different cholesterol-incubated times (50 μM). Data are presented as mean values ± S.D. One-way ANOVA with Tukey’s test. (I) MTT assay of sgCtrl/sgSNHG6 HepG2 cells at different cholesterol-incubated times (50 μM). Data are presented as mean values ± S.D. Two-way ANOVA. (J) IB detection for Lysosomes/ mitochondria/ ER quality purified from Huh7 cells. (K-M) qRT-PCR detection of SNHG6 enrichment on ER (K)/ lysosomes (L)/ mitochondria (M) in Huh7 cells. Data are presented as mean values ± S.D. (N,O) Detecting cholesterol levels in sgCtrl/ sgSNHG6 HepG2 cells (N) and shScr/ shSNHG6 Huh7 cells (O). Data are presented as mean values ± S.D. One-way ANOVA with Tukey’s test. (P) Detecting cholesterol levels in vector-/ SNHG6-Huh7 cells. Data are mean values ± S.D. Two-sided Student’s t-test. (Q,R) qRT-PCR analysis of indicated genes in shScr/shSNHG6 Huh7 cells. Data are presented as mean values ± S.D. One-way ANOVA with Tukey’s test (Q); and in Vector-/SNHG6-Huh7 cells. Data are mean values ± S.D. Two-sided Student’s t-test (R).

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