Fig. 1: In vitro effects of GLP-1RA/tesaglitazar on GLP-1R signalling.
From: GLP-1-mediated delivery of tesaglitazar improves obesity and glucose metabolism in male mice
a,b, Dose responses for ligand-induced recruitment of Nluc-tagged Mini-Gαs to hGLP-1R-GFP (a) and cAMP production in hGLP-1R+ HEK293T cells (b). c, Dose responses for ligand-induced hGLP-1R-Rluc8 internalization as measured by loss of BRET with plasma membrane marker Venus-KRAS in HEK293T cells. d–f, Dose responses for ligand-induced hGLP-1R-GFP Mini-Gαs recruitment (d), cAMP production (e) and hGLP-1R-Rluc8 internalization (f) in the mouse β-cell MIN6 cells. g, Dose responses for ligand-induced hGLP-1R-Rluc8 co-localization with the terminal lysosome marker Lamp1-mNeonGreen in HEK293T cells. h–k, Temporal resolution (h,j) and AOC (i,k) of ligand-induced (1 μM) RXR-Rluc8 and PPARγ2-YFP heterodimerization in hGLP-1R(+) HEK293T cells (h,i) and hGLP-1R(−) HEK293T cells (j,k). Data in a,c,d,g represent ± iAUC of n = 3 independent biological samples, each measured in an independent study with n = 4 technical replicates. Data in b,e,f represent ± iAUC of n = 4 independent biological samples, each measured in an independent study with n = 4 technical replicates. Data in h,i,j,k represent baseline-corrected temporal dynamics (h,j) and AOC (i,k) of n = 8 (h,i) or n = 5 (j,k) independent biological samples, each measured in an independent study with n = 4 technical replicates. Data were analysed using one-way ANOVA using the Bonferroni’s multiple comparison test. Date represent mean ± s.e.m.; asterisks indicate *P < 0.05; **P < 0.01 and ***P < 0.001. Exact P values for treatment effects are i, P = 0.0208 and P = 0.0005, k, P = 0.0008.
