Extended Data Fig. 3: Effects of BMP4 in DOX-treated IHH cells.
From: BMP4 and Gremlin 1 regulate hepatic cell senescence during clinical progression of NAFLD/NASH
(a) RT-qPCR analysis of genes involved in the YAP/TAZ pathway and inflammation in control and DOX-treated cells, stimulated with or without BMP4 (n = 4). (b) Representative immunoblots of proteins (p53, p21, and MDM2), and their quantification by densitometry (bar graphs) in siRNA-transfected IHH cells. (c-e) Bar graphs showing expression of respective proteins in control cells (with or without BMP4, n = 6) (c) and DOX-treated cells (with or without BMP4, n = 4) (d, e). Values are mean ± SEM. Statistics: One-way ANOVA followed by Bonferroni’s/Dunnett’s post-hoc test or Kruskal-Wallis with post-hoc Dunn’s test for (a, c, e), 2-tailed paired t-test for (b, d). a.u., arbitrary unit.
