Extended Data Fig. 6: BMP4 inhibits the expression of pro-inflammatory markers.
From: BMP4 and Gremlin 1 regulate hepatic cell senescence during clinical progression of NAFLD/NASH
(a) RT-qPCR analysis of CCL2, IL8, IL6, and IL-1β in LX2 cells treated with conditioned medium (50%; v/v) from control or DOX-treated IHH cells. (b) Bar graph displays BMP4 per se effects on mRNA levels of inflammatory genes (CCL2, IL-6, and IL-1β) (n = 3). (c) Quantification of IL-1β secretion in supernatant of LX2 cells treated with TNFα in presence or absence of BMP4 (n = 3). (d-f) RT-qPCR analysis of inflammatory genes (CCL2, IL-6, and IL-1β) in LX2 cells treated with TNFα (d), Etoposide (e), or DOX (f) in presence or absence of BMP4 for 48 h. Values are as mean ± SEM. Statistics: Statistical significance was determined using 2-tailed unpaired t-test for (a, f) and one-way ANOVA followed by Bonferroni’s post-hoc test for (b-e, g, h). a.u., arbitrary unit.
