Fig. 4: DOX-induced senescence in IHH cells.
From: BMP4 and Gremlin 1 regulate hepatic cell senescence during clinical progression of NAFLD/NASH
a, Representative images (bright-field) of IHH cells treated with different concentrations of DOX for 72 h (n = 3 biologically independent experiments). Scale bars represent 100 µm. b, Representative immunoblots of respective proteins in control and DOX-treated cells after 72 h (n = 4 biologically independent experiments). c, Representative immunofluorescence images of control and DOX-treated cells stained for Ki67 (green) and nuclei (DAPI, blue) (n = 3 biologically independent experiments). Scale bars represent 20 µm. d, Bar graphs showing Ki67 positivity in control and DOX-treated hepatocytes (n = 3, 7–10 randomly chosen fields from each experiment). e, Bar graphs showing the relative protein levels of senescence markers, as well as markers of the YAP/TAZ pathway, in control and DOX-treated cells, normalized to GAPDH (n = 4 biologically independent experiments). Values are mean ± s.e.m. Statistics were calculated using one-way ANOVA followed by Dunnett’s post hoc test or Kruskal–Wallis with post hoc Dunn’s test. a.u., arbitrary unit.
