Extended Data Fig. 1: Determining m6A fractions at the 18 S rRNA A1832 site in THLE-2, HepG2 and Huh7 cells using SELECT. | Nature Metabolism

Extended Data Fig. 1: Determining m6A fractions at the 18 S rRNA A1832 site in THLE-2, HepG2 and Huh7 cells using SELECT.

From: N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation

Extended Data Fig. 1

a-c, Quantification of m6A fractions at the 18 S rRNA A1832 site in THLE-2 (a), HepG2 (b) and Huh7 (c) cells. A series of indicated ratios of 18 S rRNA m6A-oligo were used to calculate the quantity of 18 S rRNA and the percentage of m6A-modified 18 S rRNA in THLE-2, HepG2 and Huh7 cells. The upper panels show fluorescence amplification curves, and the lower panels show the standard curves for calculation of m6A fractions at the 18 S rRNA A1832 site. Each point on the standard curves is indicated by the mean value from three independent experiments.

Source data

Back to article page