Extended Data Fig. 2: Determining m6A fractions at the 18 S rRNA A1832 site in patient liver samples using SELECT.
From: N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation
a-c, Upper panels: fluorescence amplification curves of the indicated ratio of 18 S rRNA m6A-oligo and 100 ng of total RNA of peritumor tissues. Lower panels: standard curves for calculation of m6A fractions at the 18 S rRNA A1832 site in peritumor tissues. Each point on the standard curves is indicated by the mean value from three independent experiments. d-f, Upper panels: fluorescence amplification curves of the indicated ratios of 18 S rRNA m6A-oligo and 100 ng of total RNA of tumor tissues. Lower panels: standard curves for calculation of m6A fractions at the 18 S rRNA A1832 site in HCC tumor tissues. Each point on the standard curves is indicated by the mean value from three independent experiments.
