Extended Data Fig. 4: ORM2 has little effects on the expression levels of genes related to fatty acid esterification and oxidation and lipid transport.
From: Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis
Related to Fig. 5. a, b, Relative mRNA level (a, n = 8 biologically independent mice per condition) and protein expression of lipogenic genes (b, n = 4 biologically independent mice per condition) in ob/ob male mice administered with Ad-ORM1, Ad-ORM2 or Ad-GFP. c-e, Relative mRNA levels of genes related to fatty acid esterification (c), fatty acid oxidation (d) and lipid transport (e) in the livers from db/db male mice administered with Ad-ORM2 or Ad-GFP. n = 6 biologically independent mice per condition. f-h, Relative mRNA levels of genes related to fatty acid esterification (f), fatty acid oxidation (g) and lipid transport (h) in the livers from adenovirus-treated HFD male mice as indicated. n = 4 biologically independent mice per condition. i-k, Relative mRNA levels of genes related to fatty acid esterification (i), fatty acid oxidation (j), and lipid transport (k) in the livers from AAV-treated HFD male mice as indicated. n = 4 biologically independent mice per condition. l-n, Relative mRNA levels of genes related to fatty acid esterification (l), fatty acid oxidation (m), and lipid transport (n) in the livers from ORM2 WT, HE and KO male mice fed a normal diet. WT and HE: n = 6 biologically independent mice per condition. KO: n = 4 biologically independent mice. o-q, Relative mRNA levels of genes related to fatty acid esterification (o), fatty acid oxidation (p), and lipid transport (q) in the livers from ORM2 WT, HE and KO male mice fed a high fat diet for 8 weeks. WT: n = 4 biologically independent mice. HE and KO: n = 6 biologically independent mice per condition. Data are shown as mean ± SEM (a, c-q). P values are determined by unpaired two-tailed Student’s t-test (c-e) and one-way ANOVA followed by Dunnett’s multiple comparisons test (a, f-q).
