Extended Data Fig. 7: ORM2 recombinant protein treatment improves liver steatosis in ob/ob mice.
From: Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis
Related to Fig. 8. a-j, ob/ob male mice were intraperitoneally injected with recombinant ORM2 protein (0.1 mg/kg, 1.0 mg/kg) or vehicle control (PBS, 0) for 10 days. a-d, Body weight (a), average daily food intake for 3 consecutive days (b), iWAT (c) and BAT weight (d). (a-d) n = 8 biologically independent mice per condition. e, H&E staining of WAT and BAT from three groups of mice. Scale bar, 50 μm. Representative result from three biologically independent mice. f-i, Liver TC (f), plasma TC (g), ALT (h) and AST (i) levels from three groups of mice. n = 8 biologically independent mice per condition. j, Relative mRNA levels of genes related to hepatic inflammation in the livers from three groups of mice. n = 8 biologically independent mice per condition. Data are shown as mean ± SEM. (a-d, f-j). P values are determined by one-way ANOVA followed by Dunnett’s multiple comparisons test (a-d, f-j).
